別稱

SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel

組織來源

皮膚

疾病

皮膚黑色素瘤

傳代比例/細(xì)胞消化

1:2傳代,消化2-3分鐘

完全培養(yǎng)基配置

MEM；10%FBS；1%P/S

簡介

SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates.

形態(tài)

星狀細(xì)胞樣

生長特征

貼壁生長

倍增時(shí)間

每周 2 至 3 次

抗原表達(dá)

Blood Type O; Rh+; HLA A2, A11, B40, Bw16

致瘤性

Yes, in nude mice; forms malignant melanoma

培養(yǎng)條件

氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。

凍存條件

凍存液：90%FBS，DMSO 10%,

或使用非程序凍存液

保藏機(jī)構(gòu)

ATCC

產(chǎn)品使用

僅限于科學(xué)研究，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。